Background: Humans and ecosystems are being harmed by the accumulation of synthetic pesticides in the environment. As a result, the use of bio-pesticides as an alternative to conventional pesticides has increased for control pests. Purpose: The investigation of both male and female rats' physiological status characteristics in relation to a single oral dose toxicity of Metarhizium anisopliae. Material and Methods: The local isolate of M. anisopliae (AUMC 5130)'s metabolic crude (MaC) and wettable powder formulation (MaF) were evaluated on Sprague Dawley male and female rats. Results: In either of the treated groups, there was no indication of a fatality or toxic effects. The results indicated that there were highly significant increase leucocyte and total erythrocyte counts of male and female treated with MaF and MaC, respectively, compared with control. Also, significant, and insignificant increase in male and female platelet count (Plt) treated with MaF and MaC, respectively. Significant, insignificant, and highly significant increase in male and female ALT, AST, and ALP, respectively, treated with both products (MaF and MaC) of the tested fungus. While lipid profile (T. cholesterol, HDL, LDL and VLDL), levels were decrease in both male and female rats treated with both MaC and MaF compared with control. There have been observed physiological changes in the body weight gain and tissue somatic index of control and all treated animals. When used as bio-insecticides, MaF and MaC products had the opposite effect on body weight gain in rats - a considerable increase - when compared to control groups.