The effect of channel aspect ratio on flow boiling characteristics (flow patterns, heat transfer and pressure drop) of HFE-7100 in copper multi-microchannel heat sinks was investigated experimentally. Three heat sinks with base area 500 mm2, channel hydraulic diameter 0.46 mm and channel aspect ratio (ratio of channel width to channel height) of 0.5, 1.0 and 2.0 were tested. The average surface roughness of the channel bottom surface was nearly the same in the three heat sinks and the measured values were 0.271, 0.286 and 0.304 μm. The local heat transfer rates were determined simultaneously with flow visualisation at mass flux ranging from 50 to 250 kg/m2s, wall heat flux from 9.6 to 191.6 kW/m2, system pressure of 1 bar and low inlet sub-cooling of 5 K. The results showed that, when the channel aspect ratio increased, the heat transfer coefficient increased, while the flow boiling pressure drop decreased. However, the heat transfer rate calculated using the heat sink base area was higher in the heat sink with the smallest channel aspect ratio, indicating an enhancement due to the largest surface area.