The major objective of this research is to create a novel mathematical model for the °ow of an electro-osmotic boundary layer in a micropolar Williamson nano°uid. This development is achieved by considering the in°uence of electro-osmotic force on an incompressible micropolar Williamson nano°uid through a Darcian °ow (Darcy model) when combined with a binary chemical reaction and the energy of activation over a linearly stretching sheet. The constituent parts of the energy equation include heat radiation, thermal and mass transport, along with joule-based heating and dissipation of viscous °uids. The problem is computationally analyzed using an equation set of nonlinear partial di®erential equations (PDEs), which are then similarly converted into a system of ordinary di®erentiation equations (ODEs). The obtained ODEs system is solved numerically using the built-in command (Parametric ND Solve) via MATHEMATICA software. A series of ¯gures are used to demonstrate numerically and graphically the in°uence of physical characteristics on the °uid behavior. The behavior of °ow is obtained by studying the streamlines around the plate in contour and three-dimensional form. In the case of non-Newtonian °uid, the results indicated that the boundary layer velocity is slower compared with the Newtonian °uid case. It is also mentioned that the graphic representation of the results shows that increasing the radiation parameter enhances thermal distribution, which means that the rate of heat transfer improved. The main ¯ndings revealed that the upward trend in the Williamson number diminishes the nano°uid °ow while enhancing the skin friction coe±cient.Also, it is discovered that as me rises, the °uid's velocity distributions in the boundary layer rise. Consequently, this type of research's signi¯cance stems from its potential uses in biomedical engineering since it could be used to dewater liquids and solids from infected human tissues.