Identification and Characterization of Glutathione S-transferase Genes in Spodoptera frugiperda (Lepidoptera: Noctuidae) under Insecticides Stress

Faculty Agriculture Year: 2023
Type of Publication: ZU Hosted Pages:
Authors:
Journal: Toxics MDPI Volume:
Keywords : Identification , Characterization , Glutathione S-transferase Genes , Spodoptera    
Abstract:
Insect glutathione S-transferases (GSTs) serve critical roles in insecticides and other forms of xenobiotic chemical detoxification. The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a major agricultural pest in several countries, especially Egypt. This is the first study to identify and characterize GST genes in S. frugiperda under insecticidal stress. The present work evaluated the toxicity of emamectin benzoate (EBZ) and chlorantraniliprole (CHP) against the third-instar larvae of S. frugiperda using the leaf disk method. The LC50 values of EBZ and CHP were 0.029 and 1.250 mg/L after 24 h of exposure. Moreover, we identified 31 GST genes, including 28 cytosolic and 3 microsomal SfGSTs from a transcriptome analysis and the genome data of S. frugiperda. Depending on the phylogenetic analysis, sfGSTs were divided into six classes (delta, epsilon, omega, sigma, theta, and microsomal). Furthermore, we investigated the mRNA levels of 28 GST genes using qRT-PCR under EBZ and CHP stress in the third-instar larvae of S. frugiperda. Interestingly, SfGSTe10 and SfGSTe13 stood out with the highest expression after the EBZ and CHP treatments. Finally, a molecular docking model was constructed between EBZ and CHP using the most upregulated genes (SfGSTe10 and SfGSTe13) and the least upregulated genes (SfGSTs1 and SfGSTe2) of S. frugiperda larvae. The molecular docking study showed EBZ and CHP have a high binding affinity with SfGSTe10, with docking energy values of −24.41 and −26.72 kcal/mol, respectively, and sfGSTe13, with docking energy values of −26.85 and −26.78 kcal/mol, respectively. Our findings are important for understanding the role of GSTs in S. frugiperda regarding detoxification processes for EBZ and CHP.
   
     
 
       

Author Related Publications

  • Ahmed Ali Ahmed Ali Ayoob, "FILED EVALUATION OF THIAMETHOXAM INSECTICIDE AND ITS MIXTURES WITH CERTAIN ADJUVANTS IN CONTROLLING LEGUMINOUS APHID, Aphis craccivora (KOCH) AND THEIR RESIDUES IN PLANTS AND SOIL", جامعة الزقازيق, 2015 More
  • Ahmed Ali Ahmed Ali Ayoob, "Reduction of soil contamination by cypermethrin residues using phytoremediation with Plantago major and some surfactants", Springier, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Insecticidal Activity of Four Lignans Isolated from Phryma leptostachya", MDPI, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Identification and analysis of full-length transcripts involved in the biosynthesis of insecticidal lignan (+)-haedoxan A in Phryma leptostachya", Elsevier, 2019 More
  • Ahmed Ali Ahmed Ali Ayoob, "Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi", Elsevier, 2019 More

Department Related Publications

  • Alsayed Mahmoud Mostafa Ibrhiem, "تأثير طرق مختلفة من الحرث والتسميد على الكثافة العددية لتعداد الحلم العنكبوتى Tetranychus urticae Koch في حقول الفول البلدي Effect of different soil tillage and fertilization regimes on population density of Tetranychus urticae Koch in fab", Egypt. J. Appl . Sci., 2009 More
  • Alsayed Mahmoud Mostafa Ibrhiem, "تأثير نوع المادة الغذائية ولون الضوء على زيادة تعداد الحلم Sancassania berlesei (Michael) (أكارى : أستيجماتا) . Food type and light colour as factors affecting population growth of Sancassania berlesei (Michael ) ( Acari : Astigmata", Journal of Agriculture Science , Mansoura University, 2008 More
  • Alsayed Mahmoud Mostafa Ibrhiem, "The harmful effects of phytophagous mite Tetranychus urticae Koch on some botanical characters of grapevine plant Vitis vinifera L. and releasing the predatory mite Typhlodromips capsicum Mostafa. التأثيرات الضارة للحلم النباتي Tetranychu", Research Journal of Agriculture and Biological Sciences, 2013 More
  • Alsayed Mahmoud Mostafa Ibrhiem, "Seasonal abundance of the two- spotted spider mite Tertranychus urticae koch on common bean Phaseolus vulgaris L.", http://www.lifesciencesite.com, 2018 More
  • Ola Ibrahim Mohamed Soliman, "Ecological Studies on Some homopterous insects infesting certain citrus and guava trees", كلية الزراعة جامعة الزقازيق, 2016 More
Tweet