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Electric field-directed migration of mesenchymal stem cells enhances their therapeutic potential on cisplatin-induced acute nephrotoxicity in rats
Faculty
Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
1077–1093
Authors:
Ghadeer Mohamed Mahmoud AbdelAal
Staff Zu Site
Abstract In Staff Site
Journal:
Naunyn-Schmiedeberg's Archives of Pharmacology Springer Nature
Volume:
Volume 396
Keywords :
Electric field-directed migration , mesenchymal stem cells
Abstract:
Cisplatin is widely used as an anti-neoplastic agent but is limited by its nephrotoxicity. The use of mesenchymal stem cells (MSCs) for the management of acute kidney injury (AKI) represents a new era in treatment but effective homing of administered cells is needed. This study aimed to investigate the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on cisplatin-induced AKI in rats after directed migration by electric field (EF). Forty-eight adult male albino rats were equally classified into four groups: control, cisplatin-treated, cisplatin plus BM-MSCs, and cisplatin plus BM-MSCs exposed to EF. Serum levels of IL-10 and TNF-α were measured by ELISA. Quantitative real-time PCR analysis for gene expression of Bcl2, Bax, caspase-3, and caspase-8 was measured. Hematoxylin and eosin (H&E) staining, periodic acid Schiff staining, and immunohistochemical analysis were also done. MSC-treated groups showed improvement of kidney function; increased serum levels of IL-10 and decreased levels of TNF-α; and increased mRNA expression of Bcl2 and decreased expression of Bax, caspase-3, and caspase-8 proteins comparable to the cisplatin-injured group. EF application increased MSCs homing with significant decrease in serum urea level and caspase-3 gene expression together with significant increase in Bcl2 expression than occurred in the MSCs group. Restoration of normal kidney histomorphology with significant decrease in immunohistochemical expression of caspase-3 protein was observed in the BM-MSCs plus EF group compared to the BM-MSCs group. EF stimulation enhanced the MSCs homing and improved their therapeutic potential on acute cisplatin nephrotoxicity.
Author Related Publications
Ghadeer Mohamed Mahmoud AbdelAal, "Electric field‑directed migration of mesenchymal stem cells enhances their therapeutic potential on cisplatin‑induced acute nephrotoxicity in rats", Springer, 2023
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Ghadeer Mohamed Mahmoud AbdelAal, "Protective Effects of Tomato Lycopene and Zingiber officinale Extracts on Gentamicin-induced Nephrotoxicity in Adult Albino Rats (Comparative study)", Zagazig University, Faculty of Medicine, Forensic Medicine and Clinical Toxicology Department, 2018
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Ghadeer Mohamed Mahmoud AbdelAal, "EARLY PHASE POSTMORTEM REDISTRIBUTION OF LIDOCAINE AND MIDAZOLAM IN ADULT ALBINO RATS", The Egyptian Society of Clinical Toxicology, 2019
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Ghadeer Mohamed Mahmoud AbdelAal, "Updates in Chemical Analysis of Drugs of Abuse", Zagazig University, Faculty of Medicine, Forensic Medicine and Clinical Toxicology Department, 2021
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