Gentamicin (GEN) is an aminoglycoside antibiotic that is widely used in treatment of gram negative bacterial infections. Tomato lycopene (LYC) and Zingiber officinaleethyl acetate extract (ZOEAE) are powerful antioxidants against free radicals and oxidative attacks. Aim of the work was to investigate the protective effects of tomato LYC and ZOEAE against GEN induced oxidative stress and apoptosis on kidney of adult albino rats. Material and methods: the study was conducted for 10 days on 72 adult albino rats of both sexes, divided into six main groups; one control (subdivided into 4 subgroups) and five treated groups (8 rats in each subgroup & group). Apart from Group І(control groups) the treated groups are treated as follow: Group ІI (LYC) treated orally with LYC (200 mg /kg/day). Group ІII (ZOEAE) treated orally with ZOEAE (200 mg /kg/day). Group IV (GEN) treated with IP injection of GEN (67.4 mg/kg/day). Group V (GEN + LYC) treated with GEN (67.4 mg/kg/day) along with LYC (200mg/kg/day). Group VI (GEN + ZOEAE) treated with GEN (67.4 mg /kg/day) along with LYC (200 mg/kg/day). All animals were sacrificed 24 hours after the last dose, their blood and kidney tissues were subjected to biochemical analysis, while the remaining kidney tissues were stained for histopathological study. Results: GEN administration for 10 days significantly increased serum urea and creatinine concomitant with marked renal histopathological changes, suggesting nephtotoxicity. Also, it significantly increased renal malondialdehyde and decreased renal reduced glutathione and catalase activity, suggesting free radical formation. GEN also caused DNA fragmentation, suggesting apoptosis. Co-administration with either tomato LYC or ZOEAE produced approximate comprehensive improvements of all studied parameters with the superiority of ZOEAE as regard histopathology. Recommendations: Supplementation with antioxidants e.g. LYC and ZOEAE in GEN treated patients had beneficial impacts through alleviating the oxidative stress and improving renal functions