Intergenerational effects from fathers to offspring are increasingly reported from diverseorganisms, but the underlying mechanisms remain speculative. Paternal trans-generational immune priming (TGIP) was demonstrated in the red flour beetleTriboliumcastaneum: non-infectious bacterial exposure of fathers protects their offspring againstan infectious challenge for at least two generations. Epigenetic processes, such as cyto-sine methylation of nucleic acids, have been proposed to enable transfer of informationfrom fathers to offspring. Here we studied a potential role in TGIP of theDnmt2gene(renamed asTrdmt1in humans), which encodes a highly conserved enzyme that methyl-ates different RNAs, including specific cytosines of a set of tRNAs.Dnmt2has previouslybeen reported to be involved in intergenerational epigenetic inheritance in mice and pro-tection against viruses in fruit flies. We first studied gene expression and found thatDnmt2is expressed in various life history stages and tissues ofT. castaneum, with highexpression in the reproductive organs. RNAi-mediated knockdown ofDnmt2in fatherswas systemic, slowed down offspring larval development and increased mortality of theadult offspring upon bacterial infection. However, these effects were independent ofbacterial exposure of the fathers. In conclusion, our results point towards a role ofDnmt2for paternal effects, while elucidation of the mechanisms behind paternal TGIPneeds further studies.