Staphylococcus aureus is a Gram positive bacterium living as a commensal on skin, mouth and upper respiratory system, making it a risk factor for opportunistic and nosocomial infections. It is the major cause of skin, bone, pneumonia, soft tissue, and urinary tract infections and other invasive infections in both the community and hospital settings. High prevalence of MethicillinResistant Staphylococcus aureus (MRSA) strains between staphylococcal isolates is very problematic. MRSA strains are common causes of nosocomial infections and are associated with increased morbidity and mortality. The aim of this study is to characterize prevalence of MRSA isolates and determine antibiotic susceptibility patterns of S. aureus clinical isolates toward various antibiotics by disc diffusion method. S. aureus isolates showed high resistance to both β-lactams and tetracycline and intermediate resistance to gentamycin, azithromycin and erythromycin. However, low bacterial resistance was noted against chloramphenicol, ciprofloxacin, clindamycin and sulphamethoxazole-trimethoprim. S. aureus isolates exhibited a higher sensitivity toward linezolid and vancomycin. The current study indicates that linezolid and vancomycin are the most effective antistaphylococcal drugs.