Metabolites Profiling and In Vitro Biological Characterization of Different Fractions of Cliona sp. Marine Sponge from the Red Sea Egypt

Faculty Pharmacy Year: 2023
Type of Publication: ZU Hosted Pages:
Authors:
Sahar Abdelaziz Mohamed Abdalaziz
Journal: molecules MDPI Volume:
Keywords : Metabolites Profiling , , Vitro Biological Characterization of Different    
Abstract:
Red Sea marine sponges are an important source of biologically active natural products. Therefore, the present study aimed to investigate, for the first time, the components of n-hexane, dichloromethane, and ethyl acetate fractions of Cliona sp. marine sponge collected from the Red Sea, Egypt using UPLC-ESI-MS/MS (Ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry) analysis. The analysis revealed the tentative identification of 23, 16, and 24 compounds from the n-hexane, dichloromethane, and ethyl acetate fractions of Cliona sp., respectively. In addition, the examination of these fractions resulted in the isolation and identification of three sterols and one amino acid. The identification of the isolated compounds was confirmed by 1D and 2D NMR (Nuclear Magnetic Resonance), and MS (Mass spectrometry), and IR (Infrared) spectroscopy. The in vitro cytotoxic, antioxidant, and antimicrobial activities of the total ethanolic extract and its sub-fractions were also evaluated. Interestingly, the ethyl acetate fraction showed potent cytotoxic activity against colon (HCT-116) and human larynx carcinoma (HEP-2) cell lines with IC (Half-maximal Inhibitory Concentration) 6.11  0.2 and 12.6  0.9 g/mL, respectively. However, the dichloromethane fraction showed strong antioxidant activity, with IC 75.53  3.41 g/mL. Notably, the total ethanolic extract showed the strongest antibacterial activity against Staphylococcus aureus and Escherichia coli, with MIC (Minimum Inhibitory Concentration) 62.5  0.82 and 125  0.62 g/mL, respectively, compared to other fractions. In conclusion, this is the first report on the secondary metabolites content and biological activities of Cliona sp. from the Red Sea, Egypt. It also highlights the need for further research on the most active fractions against various cancer cell lines and resistant bacterial and fungal strains. Cliona sp. extract and its fractions could be a potential source of novel and safe natural drugs with a wide range of medicinal and pharmaceutical applications. 50 50
    
     
 
       

Author Related Publications

  • Sahar Abdelaziz Mohamed Abdalaziz, "MACRO- AND MICROMORPHOLOGICAL STUDY OF DAUCUS CAPILLIFOLIUS GILLI CULTIVATED IN EGYPT MACRO- AND MICROMORPHOLOGICAL STUDY OF DAUCUS CAPILLIFOLIUS GILLI CULTIVATED IN EGYPT", European Journal of Pharmaceutical and Medical Research, 2018 More
  • Sahar Abdelaziz Mohamed Abdalaziz, "Anticancer activity and concurrent analysis of ursolic acid, b-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method", Elsevier, 2018 More
  • Sahar Abdelaziz Mohamed Abdalaziz, "Chemical Composition and Biological Activities of the Aqueous Fraction of Parkinsonea aculeata L. Growing in Saudi Arabia", Elsevier, 2018 More
  • Sahar Abdelaziz Mohamed Abdalaziz, "Expression of Biphenyl Synthase Genes and Formation of Phytoalexin Compounds in Three Fire Blight-Infected Pyrus communis Cultivars", PLOS ONE, 2016 More
  • Sahar Abdelaziz Mohamed Abdalaziz, "Concurrent analysis of bioactive triterpenes oleanolic acid and b-amyrin in antioxidant active fractions of Hibiscus calyphyllus, Hibiscus deflersii and Hibiscus micranthus grown in Saudi Arabia by applying validated HPTLC method", Elsevier, 2017 More

Department Related Publications

  • Ehsan Mahmoud Abdelaziz Abozied, "Pharmacognostical study and phytochemical investigation of launaea mucronata ", لايوجد, 1900 More
  • Ehsan Mahmoud Abdelaziz Abozied, "7) GC-MS and Antimicrobial Activity of Volatile Oil of Apium Leptophyllum (Benth). ", لايوجد, 1900 More
  • Ehsan Mahmoud Abdelaziz Abozied, "17) Phytochemical study and Bioassay of some constituents from Heliotropium Bacciferum Forssk", لايوجد, 1900 More
  • Ehsan Mahmoud Abdelaziz Abozied, "27) Phytochemical and Biological studies of the shells of Arachis hypogae L. Fruits (Pea Nut) Cutivated in Egypt", لايوجد, 1900 More
  • Ehsan Mahmoud Abdelaziz Abozied, "18) Macro- and Micromorphology of the Leaf, Stem & Root of Heliotropium bacciferum (Forsk). ", لايوجد, 1900 More
Tweet