Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Application of Bacteriophages for Biocontrol of Extensively Drug Resistant Salmonella Serovars Isolated from Poultry Farms
Faculty
Veterinary Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
Authors:
Mai Farag Ibrahim Abdel Razek Saad
Staff Zu Site
Abstract In Staff Site
Journal:
Journal of Advanced Veterinary Research Assiut University
Volume:
Keywords :
Application , Bacteriophages , Biocontrol , Extensively Drug Resistant
Abstract:
Salmonella is one of the most common foodborne pathogens causing diseases in humans and animals. Increased resistance to antibiotics necessitates the need for an alternative control strategy. This study aimed to screen, isolate and evaluate the bacteriophage characteristics for biocontrol of pathogenic Salmonella serovars. Twelve Salmonella isolates, including different Salmonella enterica serovars, were obtained from different sources of poultry farms. All isolates were screened for antibiotic sensitivity and showed multiple antibiotic resistance. Two lytic bacteriophages, vB_SalSph_WW1, and vB_SalM_WW2, were isolated from the sewage and characterized against Salmonella enterica serovar Enteritidis. Morphological analysis by transmission electron microscopy revealed that the vB_SalSph_WW1 phage belonged to the family Siphoviridae while the vB_SalM_WW2 phage belonged to the family Myoviridae. Both phages showed a broad host range within the Salmonella genus. Phages vB_SalSph_WW1 and vB_SalM_WW2 had a lytic effect on 3 (25%) and 4 (33.3%) of the 12 Salmonella isolates, respectively. The lytic cycle of each phage was determined by a one-step growth curve and both phages had the same short latent period (15 min). WW1 phage gave a burst size of 90 PFU/infected cell, while the vB_SalM_WW2 phage gave a higher burst size of 150 PFU/infected cell. The stability test revealed that vB_SalSph_WW1 and vB_SalM_WW2 phages were stable at pH 4–9 and pH 4–10, respectively. Both Phages exhibited high degrees of thermal tolerance with active titer as high as 42◦C. However, they lost their stability and the titers declined when heated at 50◦C for 30 min. This study revealed that vB_SalSph_WW1 and vB_SalM_WW2 phages have the potency to be used as an alternative strategy to control the infection of Salmonella in poultry farms and to prevent transmission of Salmonella infection to humans and spread of the pathogen into environment.
Author Related Publications
Mai Farag Ibrahim Abdel Razek Saad, "Occurrence, virulence, carbapenem resistance, susceptibility to disinfectants and public health hazard of Pseudomonas aeruginosa isolated from animals, humans and environment in intensive farms", Wiley-Blackwell Publishing Ltd, 2022
More
Mai Farag Ibrahim Abdel Razek Saad, "Isolation of bacteriophage as potential biocontrol agents of various E.coli serotypes", 4th International Food Safety Conference, University of Sadat City, 2017
More
Mai Farag Ibrahim Abdel Razek Saad, "Organochlorine pesticide residues in buffalo meat, liver, kidney, milk, and Kariesh cheese", Assiut University, 2023
More
Mai Farag Ibrahim Abdel Razek Saad, "Biocide Syntheses Bee Venom-Conjugated ZnO@αFe2O3 Nanoflowers as an Advanced Platform Targeting Multidrug-Resistant Fecal Coliform Bacteria Biofilm Isolated from Treated Wastewater", MDPI, 2023
More
Mai Farag Ibrahim Abdel Razek Saad, "Residual contents of the toxic metals (lead and cadmium), and the trace elements (copper and zinc) in the bovine meat and dairy products: residues, dietary intakes, and their health risk assessment", Taylor & Francis, 2021
More
Department Related Publications
Alsadek Khaliel Youssef Sliem, "Effect of management regime on behaviour and welfare of calves ", لايوجد, 1900
More
Amro Mohamed Mohamed Abdelaal , "تأثير بعض المطهرات الكيميائية شائعة الاستخدام و الأشعة فوق البنفسجية على خمسة من الملوثات الفطرية ذات الاهمية البيطرية والصحية واسعة الانتشار فى مساكن الماشية", لايوجد, 1900
More
Mohamed Abdelwahab Ahmed Areaf , "collaborative study of some screening test for detection of mastitis milk", لايوجد, 1900
More
Mohammed Youssef Ibrahim, " Behaviour of Arabian foals in relation to system of rearing. ", لايوجد, 1900
More
Mohammed Youssef Ibrahim, "Effect of management regime on behaviour and welfare of calves. ", لايوجد, 1900
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف