Aim: Assessment of vitamin D status in Hemophilia patients at Zagazig University Children Hospitals. Subjects& Methods: The study was carried out at the Pediatrics and Clinical Pathology Departments/ Zagazig University Children Hospitals (ZUCH) during the period from February 2018 to August 2018. The study was performed on 30 Hemophilia A male children aged from 6 to 18 years old. Participants were registered in and were followed up regularly, at the Pediatric Hematology Outpatient Clinics. Participants were subjected to medical history taking, assessment of the body mass index (BMI) and investigations that included Ca, Ph, ALP, vitamin D. Statistical analysis for obtained data using suitable significance tests was performed. Results: 53.3 % of Hemophilia patients showed marked factor VIII deficiency compared to 33.3% who showed moderated deficiency. 26.7% of all patients showed deficiency of vitamin D (10-19.99 ng/dl) compared to 40% who had severe deficiency of vitamin D (level <10 ng/dl). Conclusion: There was a statistically significant direct proportion between both factor VIII and vitamin D deficiencies as the severer factor VIII deficiency, the lower vitamin D level.