The present study was conducted at two field experiments in a private farm at Gilbana Zone in Sinai, East Qantarah District, Ismailia Governorate, Egypt, during the two successive winter seasons 2015/2016 and 2016/2017. This investigation aimed to find out the effect of nitrogen fertilizer levels (15, 30, 45 and 60 kg N/fad.), barley seeding rate (40,60 and 80 kg/fad.) and nano-fertilizer application on growth, yield and its components as well as grain quality of barley. The spilt-spilt plot design as a form of the randomized complete block design with three replications was followed. Application of nano-fertilizer appeared to produce high values in plant height, flag leaf area, No. of tillers/plant, spike length, No. of both spikelets and grains per spike, grain protein content . Also , nano-fertilizer supply coused superiorty in each of grains/spike, 1000- grain weight, harvest index, grain yield/fad. and biological yield/fad. Seeding rate of 80 kg/fad. appeared to produce the tallest plants, the highest value in each of number of spikes/m2, grain yield/fad. and biological yield/fad. While the middle seeding rate of 60 kg/fad. gave the highest harvest index. Meanwhile, the seeding rate of 40 kg/fad. recorded the highest values in each of No. of both spikelets and grains/spike, weight of both grains/spike and 1000-grain. Nitrogen fertilization was very effective on all yield characteristics, so any increase in N level was followed by a significant increase in plant height, flag leaf area, No. of tillers/plant, spike length, No. of spikelets/spike, grains/spike and spikes/m2, also increased weights of both grains/spike and 1000-grain as well as grain and biological yields/fad. and harvest index as well as protein and carbohydrate contents in grains. These results finally recommend sowing barley with dense seeding rate of 80 kg/fad. and application of 60 kg N/ fad. In addition to application of nano-fertilizer to maximize grain yield /fad. under the experimental site and other likely environmental conditions.