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Characterization of two novel lytic bacteriophages for reducing biofilms of zoonotic multidrug-resistant Staphylococcus aureus and controlling their growth in milk
Faculty
Veterinary Medicine
Year:
2020
Type of Publication:
ZU Hosted
Pages:
Authors:
Asmaa Salah Mouhamd
Staff Zu Site
Abstract In Staff Site
Journal:
LWT-Food Science and Technology Elsevier
Volume:
Keywords :
Characterization , , novel lytic bacteriophages , reducing biofilms
Abstract:
This study targeting the characterization of new lytic phages and studying their impact on Staphylococcus aureus biofilm and growth in milk. Two lytic phages (vB_SauM_ME18 & vB_SauM_ME126) were isolated using 40 Multidrug-Resistant (MDR) S. aureus isolates. Four isolates (10%) were susceptible to phages. Phages were related to Family Myoviridae, had icosahedral heads and long contractile tails. The genome size is 20 kbp for both phages and DNA was digested with hinf I, hind III and hae III. Phages had a latent period (~15 min), rise period (~30 min) and burst size (114 PFU/infected cell for ME18 and 140 PFU/infected cell for ME126). There was a significant reduction in biofilm formation and reduction in the established biofilm biomass in phage treated S. aureus compared with controls (P < 0.05). Phages showed high activity and eliminated MDR S. aureus from ultra-heat-temperature treated milk at 25 °C. However, at 37 °C, ME126 (MOI10) eradicated S. aureus and ME18 (MOI10) reduced the CFU/ml by 87.2% compared with controls (P < 0.05). In essence, lytic phages are promising natural antimicrobials for inhibiting the growth and biofilm of MDR S. aureus in milk, thereby, biocontrol of foodborne pathogens.
Author Related Publications
Asmaa Salah Mouhamd, "Occurrence of Bacillus cereus in raw milk and some dairy products in Egypt", Japanese Journal of Veterinary Research 64(Supplement 2): S95-102, 2016., 2016
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Asmaa Salah Mouhamd, "Diversity of lactic acid bacteria isolated from raw milk inElsharkia province, Egypt", Japanese Journal of Veterinary Research 64(Supplement 2): S23-30, 2016., 2016
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Asmaa Salah Mouhamd, "Effect of milking techniques on milk composition and sanitary condition of milk in El Sharkia Governorate", Journal of the Egyptian Veterinary Medical Association., 2013
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Asmaa Salah Mouhamd, "Prevalence, molecular characterization and antimicrobial resistance of L. monocytogenes isolated from raw milk and dairy products.", Zagazig university, 2014
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Asmaa Salah Mouhamd, "SCREENING OF SOME DAIRY PRODUCTS IN EGYPT FOR AFLATOXIN-M1 CONTAMINATION AND SOME HEAVY METAL RESIDUES.", Springer, 2017
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