Abstract : Background: Cryopreservation of human ovarian tissue has been widely used all over the world to protect fertility, not only for women undergoing chemotherapy, but also for women who wish to delay pregnancy for a certain period in order to preserve fertility. Aim of the study was to find out the effect of an educational program on knowledge and attitude of female students' regarding oocyte cryopreservation. Research Design: quasi-experimental research design was used in this study. Setting: This study was conducted at faculties of art and education, Zagazig University, Egypt. Sample: purposive sample composed of 115 of postgraduate female students. Results: After implantation of an educational program 97.2% of study subject shows highly satisfactory score of total knowledge compared to 12.3% in preintervention phase. As well as 92.3% of study subject reported positive attitude in posttest compared to 25.3% in pretest. Conclusion: The educational program was effective on increase knowledge and change attitude of post graduated female students regarding oocyte cryopreservation. Recommendation: More efforts needed by the health care system, to increase the youth knowledge about ways to preserve their fertility especially unmarried girls Keywords: post graduate female, Knowledge, Attitude, oocyte cryopreservation