Background: The problem of how to properly dispose of healthcare waste has been a serious challenge. The aimof this research was to evaluate the effect of an educational program for nurses working in Maternal and Child HealthCare Centers about health care waste management. Design: A quasi-experimental design was used. Settings: Thisresearch took place at 16 Maternal and Child Health Care Centers in Zagazig City, Sharikia Governorate, Egypt, which were chosen randomly from 34 Maternal and Child Health Care Centers and all nurses in MCHcenters, wasrecruited for this study. Sample: A simple random sample of 99 nurses from the Maternal and Child HealthCareCenters. Tools: Two tools were used for data collection. Tool I: Data were collected about nurses' socio- demographic characteristics and their knowledge about waste management before and after the programusing a self- administered knowledge questionnaire. Tool II: To evaluate nurses' waste management practices usinganobservation checklist. Results: The total good knowledge level in pre-intervention was 57.6%, which improvedto94.9% after one month of intervention. Furthermore, the total score of nurses' practices increased from28.3%at preintervention to 71.7% after one-month post-intervention. Conclusion: It was concluded that the educational programeffectively improved nurses’ knowledge and practices, while the practice concerning controlling health care wasteisstill inadequate. There was a highly statistically significant positive linear correlation between the nurses’ knowledge and reported practices regarding the management of healthcare waste before and after applyingtheeducational program p<0.01. Recommendations: Pre-employment health education programs should be providedfor Egyptian nurses on biomedical waste management with orientation to organization, and they must be checkedregularly