Background: Lack of knowledge regarding polycystic ovary syndrome (PCOS) and poor lifestyle is considered to be the major factor leading to polycystic ovary syndrome. Aim of the study: To evaluate lifestyle and reproductive health in female nursing students about polycystic ovary syndrome. Subjects and Methods; Research design: A descriptive design was utilized in the present study. Setting: The study was carried at Faquos nursing technical institute from the first of November 2022 to the end of February 2023. Subjects: A convenient sample was used in the study (200 females nursing students). Tools of data collection: A structured interview tool, lifestyle assessment tool and reproductive health knowledge assessment tool were used for data collection. Results: 35.0% of female nursing students had healthy lifestyle and 65% had unhealthy lifestyle. 22.5% of female nursing students had satisfactory level of total reproductive health knowledge and 77.5 had unsatisfactory level of total reproductive health knowledge. There was high significant statistical positive correlation between female nursing students’ lifestyle and knowledge. Conclusions: There was unhealthy lifestyle and unsatisfactory level of total knowledge regarding PCOS. Polycystic ovary syndrome is a condition which can lead to severe health related problems and affects female’s reproductive health if not treated properly. Recommendations: Application of educational program for adolescent girls in different setting should be conducted in order to improve their knowledge, enhance lifestyle and allow immediate recognition regarding PCOS through continuous counseling, support, and encouragemen