Molecular biology and physiology: Ty3/gypsy retro-transposons in Egyptian cotton (G. barbadense)

Faculty Technology and Development Year: 2024
Type of Publication: ZU Hosted Pages:
Authors:
Journal: The journal of cotton science food and agriculture organization Volume:
Keywords : Molecular biology , physiology: Ty3/gypsy retro-transposons , Egyptian    
Abstract:
The objective of this research was to isolate and characterize Ty3/gypsy sequences in culti-vated allotetraploid cotton, Gossypium barbadense L. Using a degenerate oligonucleotide designed to amplify these sequences by PCR, part of the reverse transcriptase gene of Ty3/gypsy retro-transposons was isolated, cloned, and sequenced. These retro-elements exist in the cotton genome in high copy number, and their encoded transcripts were detected. Phylogenetic analysis shows that these retro-elements are heterogeneous and closely related to elements present in other plant species. These results indicate that Ty3/gypsy retro-transposons are standard component of the Gossypium genome, and support the fact that Ty3/gypsy retro-transposons represent a major component of the plant genome. R etro-elements and their derivatives are ubiquitous
   
     
 
       

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