Recently, a brand-new combination of mirabegron and solifenacin succinate received approval for managing the overactive bladder syndrome's bothersome symptoms. Herein, we introduce six validated UV-spectrophotometric approaches relying on simple mathematical calculations for rapid and concurrent analysis of MBR and SOS in their synthetic mixtures, commercial tablets, and human urine without preliminary separation. The proposed approaches were dualwavelength, induced dual-wavelength, Fourier self-deconvolution, area under the curve, ratio difference, and first derivative ratio methods. These approaches were applied successfully for the concurrent determination of MBR and SOS in their mixtures (suffered from severe spectral overlap) with excellent recoveries without interference from pharmaceutical excipients or biological matrices. The concurrent analysis of MBR and SOS in spiked biological fluids was accomplished for the first time in this study using the Fourier approach. The proposed approaches were linear in ranges of (50–450) μg/mL and (70–1200) μg/mL for MBR and SOS, in order. The proposed approaches' greenness was evaluated using two tools namely; penalty points scoring system and green analytical procedure index (GAPI), revealing their superiority in greenness over the reported LC method. The proposed approaches were compared statistically in terms of t- and Fvalues with the reported LC method, revealing no discernible differences concerning accuracy or precision at p = 0.05. Accordingly, the proposed approaches are valid for regular analysis of MBR and SOS as affordable substitutes in QC labs lacking expensive chromatographic instruments