Abstract: |
SUMMARY
This study was carried out in private farms at sharkia province and in the abattoir of Zagazig city.
This work included three experiments:
1-1st experiment "Effect of tethering in maintenance behaviour and performance of yearling Friesian bull":
The obtained results were included that:
There was no a significant difference in eating and drinking times between tethered group and loosed group.
The tethered animal spent more time in idling, lying & rumination than loosed one.
There was no significant difference in frequencies of defecation, urination, kicking & self grooming between tethered and loosed animals.
There was a significant difference (P < 0.05) in frequencies of heading, butting, mutual grooming between tethered and loosed group.
The mean frequencies of oral stereotypes were greater in tethered bull in compare with loosed one.
There was a significant difference in plasma cortisol levels and body weight gain between tethered and loosed groups.
2-2nd experiment "Effect of the feeding time change in maintenance behaviour & performance of yearling bull":
The results of experiment included that:
There was a significant difference (P < 0.05) in eating, drinking, idling & lying times between regular feeding time and irregular feeding time.
There was no significant difference in rumination time between experimental groups.
Mean frequencies of defection, bar biting and tongue rolling in regular feeding time were higher than irregular feeding time.
There was no significant difference in frequencies of urination, kicking, heading, butting and mutual grooming in experimental groups.
There was a significant difference (P < 0.05) in body weight gain experimental groups.
The mean level of plasma cortisol was the highest in irregular feeding time.
3-3rd experimental " Meat quality & plasma cortisol in relation to pre-slaughter transportation and handling":
The obtained results were:
In the present study, a total of 180 (129 nasal swab and 51 lung tissue) samples were collected from both imported and native cattle and buffalo from different localities of Sharkia Governorate. Only 88 out of 180 samples were screened by sandwich ELISA commercial kits for detecting BRSV antigen. Only one lung tissue sample was positive obtained from a buffalo with a percentage of 1.1 %.
All collected samples were used in isolation attempts on MDBK cells. As shown in Table (4), the virus was isolated from 35 samples out of 180 tested samples on MDBK cells with a percentage of 19.4 %. There were 27 samples out of 134 tested imported cattle samples were isolated, (2 from lung tissues and 25 from nasal swabs) with a percentage of 20 % and 8 samples out of 34 tested buffalo samples, (5 from lung tissues and 3 from nasal swabs) with a percentage of 23.5 %, but not isolated from native cattle. These results indicated that the imported cattle and buffalo were more susceptible to RSV infection than native cattle.
The percentage of samples that showed CPE increased with the number of passages. It was found that some samples were not isolated by the first passage, but it needs 3-5 passages to be isolated on MDBK cells.
Also all collected samples were subjected to direct fluorescent antibody technique for identification of BRSV antigen. As shown in Table (5), the virus isolate was detected in 39 samples out of 180 tested samples with percentage of 21.6%. There were 2 virus antigens were detected in out of 12 tested native cattle samples with a percentage of 16.7 %, in 29 samples out of 134 tested imported cattle samples with a percentage of 21.6 % and in 8 samples out of 34 tested buffalo samples with a percentage of 23.5 %.
Our results concluded that BRSV was prevalent among imported cattle and Egyptian buffalo in Sharkia Governorate.
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