Background: Ova cryopreservation is a crucial component of assisted reproductive technology; it is required for fertility preservation, infertility treatment, and reproductive biology. Aim of the study: was to evaluate effect of educational program on nursing students’ beliefs and barriers regarding ova cryopreservation. Research design: Quazi experimental design was adopted to carry out this study. Setting: The study was conducted at faculty of nursing, at Zagazig University. Subjects: consisted of 200 nursing students in a multistage cluster random sample. Tools of data collection: Three tools were used for data collection. Tool I: A Structured Interviewing questionnaire, Tool II: Beliefs Assessment Tool and Tool III: Barriers Assessment Tool. Results: Following the implementation of the teaching program, there was a noticeably positive change in the overall students' beliefs on ova cryopreservation, with a highly statistically significant difference at (P < 0.001). Additionally, a highly statistically significant difference at (P < 0.001) indicated a significant improvement in the overall students' barriers about ova cryopreservation after the implementation of the teaching program. Conclusion: it was concluded that; there was highly statistical significant improvement regarding total nursing students’ beliefs and barriers regarding ova cryopreservation after implementation of educational program. Also there were high significant statistical positive correlation between students’ beliefs and barriers regarding ova cryopreservation at pre and post implementation of educational program (p < 0.001). Recommendations: All students should have access to a concise and in-depth handbook about ova cryopreservation, and there is a need to support and enhance the infrastructure for introducing services and providing financial support for fertility preservation. Key words: Ova cryopreservation, reproductive biology, fertility preservation, beliefs, barriers