This study aimed to identify the effect of some risk factors on prevalence of endometritis in dairy herds, assess the efficacy of Ceftiofur antibiotic in treatment of clinical endometritis, determine the efficacy of various diagnostic tests in diagnosis of subclinical endometritis and make a recommendation for a valuable field test for forecasting and screening of subclinical endometritis in herds. A total number of 91 Holstein Friesian cows aged from 3 to 5 years and of different parities were enrolled in this study. We used rectal examination, vaginal examination, Cytological examination, Reagent test strips, Serum analysis and treatment of clinical endometritis cases with ceftiofur. The prevalence of CE (N= 15) is 16.48% against 37.36% for SE (N= 34). Healthy cows were distinguished from SE group by counting PMN%. Healthy cows represent the control group (N= 42 with a percentage of 46.15%). Plus, excluding 19 cows that suffered from a poor BCS, mastitis, lameness, dystocia, RFM, acute puerperal metritis, or unknown postpartum fever. From the results, male calves contributed to more CE cases than female calves. In more detailed words, among the male parturitions there was 26.09% dams suffered from CE versus 6.67% among who gave females calves. Regarding the season of parturition, incidence of CE and SE was related to cold weather more than hot one. The association between these two factors may be explained by the fact that cows' general health declines during the wet seasons, leaving them more susceptible to uterine infections. We have found no significant relationship between parity of cow and incidence of endometritis. Because in our study we excluded all cows that had an abnormal parturition or that required major calving assistance. Also, the herd under study is relatively younger than those in other studies, providing less parities, hence, a less cumulative effect on uterine health, elasticity and involution capacity. Using ceftiofur to treat and prevent clinical endometritis in postpartum dairy cows did not have any advantages. It can be assumed that ceftiofur concentrations did not go above the minimal inhibitory concentrations needed to get rid of uterine infections. The best minimally invasive diagnostic technique for the identification of SE is endometrial cytology using CB. At cutoff value of 5% PMN, our data showed that SE cows recorded PMN% exceeding 5% at both 21 and 45 DIM, and control group recorded a ratio lower than 5% with a significant difference between the two groups. There was positive correlation between results of pH and cytological endometritis. Meanwhile, for LE results, it was correlated at 21 DIM but the value declines till reaches no significant difference comparing with control group at 45 DIM. Indicating that LE is more reliable at early post-partum stage than late one. On the other hand, Protein value wasn’t reliable at 21 DIM, but it showed some significance at 45 DIM. Protein reagent strip test could not predict future fertility and showed the least correlation with cytological endometritis. The results reported that in pre-partum stage, albumin concentration in control group was (3.04±0.03 g/dl) which is significantly higher than (2.07±0.03 g/dl) in SE group. Lower albumin can be an indicator of fatty liver infiltration, which is a known risk factor for uterine disorders. So, Albumin measurement is considered a reliable prognostic and diagnostic tool for subclinical endometritis. High serum Hp concentration in the postpartum period is related to a higher incidence of pre- and postpartum problems. We have found a slight difference in Hp concentration between SE and healthy groups in pre-partum stage. In fact, regardless of the difference, this result is tricky. Because of the narrow difference detected making us doubtful to depend on it as prognostic tool for endometritis. Thus, Hp can be used as benefit diagnostic tool up to 21 DIM, but not a prognostic test.