Zagazig University Digital Repository
Home
Thesis & Publications
All Contents
Publications
Thesis
Graduation Projects
Research Area
Research Area Reports
Search by Research Area
Universities Thesis
ACADEMIC Links
ACADEMIC RESEARCH
Zagazig University Authors
Africa Research Statistics
Google Scholar
Research Gate
Researcher ID
CrossRef
Biodegradable biliary stents coated with mesenchymal stromal cells in a porcine choledochojejunostomy model
Faculty
Medicine
Year:
2023
Type of Publication:
ZU Hosted
Pages:
Pages 483-489
Authors:
Staff Zu Site
Abstract In Staff Site
Journal:
Cytotherapy Elsevier
Volume:
Volume 25
Keywords :
Biodegradable biliary stents coated with mesenchymal
Abstract:
Background aims Roux en y anastomosis is a preferred method of biliary reconstruction in liver transplantation that involves living donors or pediatric patients. However, biliary stricture is a frequent and serious complication, accounting for up to 40% of biliary complications in these patients. Previously, we demonstrated that extraluminal delivery of adipose-derived (AD) mesenchymal stromal cells (MSCs) decreased peri-biliary fibrosis and increased neo-angiogenesis in a porcine model of duct-to-duct biliary anastomosis. In this study, we used a porcine model of Roux en y anastomosis to evaluate the beneficial impact of a novel intraluminal MSC delivery system. Methods Nine animals were divided into three groups: no stent (group 1), bare stent (group 2) and stent coated with AD-MSCs (group 3). All animals underwent cholecystectomy with roux en y choledochojejunostomy. Two animals per group were followed for 4 weeks and one animal per group was followed for 8 weeks. Cholangiograms and blood were sampled at baseline and the end of study. Biliary tissue was collected and examined by Masson trichrome staining and immunohistochemical staining for MSC markers (CD34 and CD44) and for neo-angiogenesis (CD31). Results Two of three animals in group 1 developed an anastomotic site stricture. No strictures were observed in the animals of group 2 or group 3. CD34 and CD44 staining showed that AD-MSCs engrafted successfully at the anastomotic site by intraluminal delivery (group 3). Furthermore, biliary tissue from group 3 showed significantly less fibrosis and increased angiogenesis compared with the other groups. Conclusions Intraluminal delivery of AD-MSCs resulted in successful biliary engraftment of AD-MSCs as well as reduced peri-biliary fibrosis and increased neo-angiogenesis.
Author Related Publications
Department Related Publications
Ahmed Alsayed Ahmed Lashen, "تصليح الفتق الاربى " محاولة اليجاد طريقة جديدة", لايوجد, 1900
More
Ahmed Mosaad Ibrahiem Nagieb, "افرازات حلمة الثدي اهمية العينة الجرالحية لحالات افرازات حلمة الثدي ذات القيمة الجراحية", لايوجد, 1900
More
Ahmed Mosaad Ibrahiem Nagieb, "استئصال القناه الابنه بسبب افراز من قناة بجملة الثدي باستعمال طريقة جراحية جديدة لتحديد بواسطة الحقن وعادة افيدون ", لايوجد, 1900
More
Ahmed Mosaad Ibrahiem Nagieb, "تشخيص التهاب الزائدة الدودية بالتصوير الفوق صوتي ومقارنة بالواحدة السريرية والمعطيات الجراحية", لايوجد, 1900
More
Osama Hassan Ghareeb Ibrahiem, "دراسة مقارنة بين طريقة بوجوتا وطريقة الشفط مع الحشرى فى علاج متلازمة المتجزئة الباطنية الناتجة من ارتفاع الضغط داخل البطن", لايوجد, 1900
More
جامعة المنصورة
جامعة الاسكندرية
جامعة القاهرة
جامعة سوهاج
جامعة الفيوم
جامعة بنها
جامعة دمياط
جامعة بورسعيد
جامعة حلوان
جامعة السويس
شراقوة
جامعة المنيا
جامعة دمنهور
جامعة المنوفية
جامعة أسوان
جامعة جنوب الوادى
جامعة قناة السويس
جامعة عين شمس
جامعة أسيوط
جامعة كفر الشيخ
جامعة السادات
جامعة طنطا
جامعة بنى سويف