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Morphological, Biochemical and Molecular Characterization of Rhizobia of Faba Bean Plants Grown in North Nile Delta Egypt
Faculty
Agriculture
Year:
2021
Type of Publication:
ZU Hosted
Pages:
Authors:
Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty
Staff Zu Site
Abstract In Staff Site
Journal:
Pakistan Journal of Biological Sciences Pakistan Journal of Biological Sciences
Volume:
Keywords :
Morphological, Biochemical , Molecular Characterization of Rhizobia , Faba
Abstract:
Rhizobia are bacteria including genes codes for enzymes involved in the fixing of the atmospheric nitrogen. A set of twenty rhizobial isolates were studied to determine their morphological, biochemical, molecular characteristics using the 16S rRNA gene in addition to assess their growth and symbiotic performance. Rhizobial isolates were isolated from root nodules of Vicia faba L. plants. The isolates were morphologically characterized by determining cell shapes, size, Gram stain reaction, motility, sporulation, bacterial growth performance was determined by IAA production and biomass density. Symbiotic performance was measured by evaluation of nodulation status and shoot/root dry weight. Sequencing of 16S rRNA and phylogenetic analysis were done for the five promising isolates. Statistical analysis was performed using a one-sample Student t-test. Only five rhizobial isolates (Rh 32, Rh 6-A, Rh 3-4, Rh RL3 and Rh 8-A) were selected according to their growth and symbiotic performance and subjected to further molecular characterizations. All isolates were found to have remarkable nodulation status, IAA production, nitrogenase activity and increasing the root and shoot dry weight. The five selected rhizobial isolates were identified by partial sequencing of 16S rRNA genes and registered in the GenBank database. The alignment and phylogenetic analyses of 16S rRNA sequences closely related in the GenBank revealed that all isolates belonging to Rhizobium leguminosarum bv. viciae. The results confirmed that the five Rhizobial strains will be promising as a source of genes for nitrogen fixation and plant growth promotion.
Author Related Publications
Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty, "IDENTIFICATION OF MOLECULAR MARKERS FOR SALT AND DROUGHT TOLERANCE USING BULKED SEGREGANT ANALYSIS (BSA) IN BREAD WHEAT (Triticum aestivum L.)", Zagazig Journal of Agricultural Research, 2014
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Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty, "Genome‑wide identification and expression analysis of metal tolerance protein (MTP) gene family in soybean (Glycine max) under heavy metal stress", Molecular Biology Reports, 2023
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Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty, "RNA sequencing in Artemisia annua L explored the genetic and metabolic responses to hardly soluble aluminum phosphate treatment", Functional & Integrative Genomics, 2023
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Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty, "Assessment of Eight Faba Bean (Vicia faba L.) Cultivars for Drought Stress Tolerance through Molecular, Morphological, and Physiochemical Parameters", Sustainability, 2023
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Ahmed Salaheldeen Mohamed Abdelrahman Eldomyaty, "Assessment of the R2R3 MYB gene expression profile during tomato fruit development using in silico analysis, quantitative and semi-quantitative RT-PCR", Saudi Journal of Biological Sciences, 2022
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Department Related Publications
Ahmed Mansour Mohamed Mansour Elzohery, "Genes, Genomes and Genomics", Global Science Books, Ltd, 2010
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Ahmed Mansour Mohamed Mansour Elzohery, "Bio-fertilizer and organic manure affects rice productivity in newly reclaimed saline soil.", Dynamic Soil, Dynamic Plant 2009, 2009
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Ahmed Mansour Mohamed Mansour Elzohery, "From PCR to the Bioinformatics Era and Integrative Biology The Digitalization of Life", Global Science Books, Ltd, 2009
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Ahmed Mansour Mohamed Mansour Elzohery, "Gene-Tracer: Algorithm Tracing Genes Modification from Ancestors through Offsprings", International Journal of Computer Applications (IJCA), 2012
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Ahmed Hosieny Abdou Mhamd, "Nile Tilapia as bio indicator to estimate the contamination of water using SDS-PAGE and RAPA-PCR techniques", faculty of Agr. Ain Shams University, 2012
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