Background: Glycated hemoglobin (HbA1c) is the parameter of choice to evaluate the long term degree of glycaemic control in patients with diabetes mellitus (DM). Iron Deficiency Anemia is the most common cause of anemia. Objective: We study the effect of iron deficiency anemia on HbA1c levels in non-diabetic individuals. Patients and Methods: This was a case-control study in the period from February 2019 to September 2019. Included 90cases at outpatient Clinic of Internal Medicine Department in Zagazig University Hospitals. The patients were randomly divided (by alternation) into three groups 30 patients, Group1: who are healthy. Group2: who have iron deficiency anemia. Group3: who has any type of anemia other than iron deficiency anemia. A detailed history of clinical examination and biochemical examination was performed including HbA1c. Results: The study revealed that there was a highly significant difference between the before and after treatment in the iron deficiency group as regard HbA1c and there was no significant difference between the studied groups as regard FBG or PPG. Conclusion: There is a significant relation between HbA1c and iron deficiency anemia while there is no relation between iron deficiency anemia and Fasting blood sugar or Postprandial sugar, Iron deficiency anemia elevates HbA1c levels in non-diabetic individuals.