The aim of this study to investigate the effect of replacement of wheat flour with barley and oat flour on the physicochemical and sensory properties of the snacks and the effect of this product on some biochemical parameters of kidney and liver in nephrotoxicity induced by GM in rats were investigated. Snacks recipes were prepared; using 100% wheat flour, 50% replacement levels of wheat flour (WF) by oat flour (OF) and 50% replacement levels of wheat flour (WF) by barley flour (BF). The results showed that the snacks produced from replacement levels of wheat flour (WF) by 50% of barley and oat flour gave the highest scores of sensory properties than the control. Snacks containing 50% oat and 50% barley flour were used to feed rats with gentamicin-induced nephrotoxicity: The rats used in the study were randomly divided into 5 groups. Each group had 8 rats :(group 1) fed on basil diet and served as control , (group 2) fed on basil diet with injected with GM, (group 3) , rats fed on basil diet treated with gentamicin with 20 % wheat flour snacks, (group 4) rats fed on basil diet treated with gentamicin with 20 % snacks containing 50 % oat flour (OF), and (group 5) rats fed on basil diet treated with gentamicin with 20 % snacks containing 50 % barley flour (BF). Blood samples were taken after 24 hours from the end of experiment which lasted eight days. The results showed that the introduction of snacks containing oats and barley into the meal of rats treated with gentamicin for 6 weeks induced significant (P< 0.05) decreases in serum urea nitrogen, creatinine, ALT, AST, ALP, serum total cholesterol and triglycerides when compared with GM- intoxicated rats. The nephroprotective mechanisms of barley and oat could be attributed to inhibition of lipid peroxidation and enhancement of kidney and liver functions. These results affirm the traditional use of these materials in folk medicine for the prevention of kidney diseases.