The research aims to identify the effect of the use of water Pilates exercises and its impact on the level of estrogen hormone, the level of depression and life satisfaction in pre-menopausal women. The two researchers used the experimental method for one group, the research community of women participating in the Egyptian Plaza Club in Zagazig, where they numbered (30) women, and the basic research sample was chosen by the intentional method. Where the number of them reached (15) women from the total research community after excluding (2) women who had a medical disease, (3) women because they did not want to participate, their ages ranged between (45: 55) years, and (10) women were selected to conduct the exploratory study. Among the research tools: the socioeconomic level scale, the Aaron Back scale for depression, the Arabization of Abdel Sattar Ibrahim, the life satisfaction scale prepared by Magdi El-Desouki, the Pilates training program. The most important results were: 1- The practice of water Pilates exercises contributed to improving the level of estrogen hormone in the research sample. 2- The practice of water Pilates exercises reduces the feeling of depression and improves the mood of women in the pre-menopausal stage. 3- The practice of water Pilates exercises contributed to improving the level of life satisfaction among the research sample. 4- Water Pilates exercises contribute to a decrease in the level of depression in pre-menopausal women, which leads to a higher satisfaction with life and an improvement in their mental health.