Background: Non-invasive fetal wellbeing is crucial to prevent fetal and maternal distress, especially in high risk pregnancies. To promote the initiatives aimed at reducing fetal death, the maternity nurses must be skilled in performing different fetal wellbeing assessment methods. Aim of the study: Evaluate the effect of educational program on maternity nurses’ knowledge and practices regarding Non-invasive fetal wellbeing assessment methods. Subjects and method: Research Design: - A quasi-experimental research design with pre/posttest and follow up was used. Setting: The present study was conducted at the fetal wellbeing assessment unit at antenatal outpatient clinic affiliated in Zagazig University hospitals. Subject: A convenient sample of all available 35 nurses who were working in the previous mentioned settings. Tools of data collection: Two tools were used: Tool I: A structured interviewing sheet containing two parts: Part I: Demographic characteristics of nurses and part II: Nurse’s knowledge questionnaire concerning non- invasive fetal well beings’ assessment methods and Tool II: Fetal wellbeing procedures practice checklist. Results: it was indicated that, there were highly statistically significant variations in nurses' knowledge & practices pre, post and throughout the follow-up phase. In addition, there was a statistically significant correlation between total level of knowledge and total level of practice during post & follow up phase. Conclusion: There was a significant improvement of maternity nurses’ knowledge and practices. The educational program positively significantly affected on maternity nurses’ knowledge and practices towards non- invasive fetal wellbeing assessment methods. Recommendations: A regular planned in-service training programs for both new and current maternity nurses should be applied to improve their performance regarding non-invasive fetal wellbeing, assessment methods. Keywords: Educational program, Maternity