Malaria is the most prevalent parasitic endemic disease in North Sudan, 75% of the total population is at risk. WHO recommends the use of insecticide-treated nets (ITNs) an effective malaria control strategy. This study was aimed to assess the knowledge, attitudes and practices of mother’s about Insecticide-Treated Nets as one of the preventive measures against malaria. This is a descriptive community based study of (295) mothers living in Abo Harira village in North Sudan. The pre-tested structured questionnaire was used for data collection. Multivariate logistic regression was used to study association between the dependent and independent variables, using Spss version 20. The study showed that more than half (55.9%) of mothers had good knowledge regarding ITNs, In spite of good knowledge about ITNs, (66.8 %) of mothers still had negative attitude and only (27.8%) reported always sleeping under it. Multivariate analysis suggested that mothers aged ≤31 years were more likely to have good knowledge about ITNs compared with mother with age ≥ 32 years [OR; 0.5174 (95% CI: 0.2974-0.9001)]. Similarly, mothers who had formal education were more likely to have knowledge about ITNs, two time higher than those with informal education [OR; 2.2 (95% CI: 1.274-3.788)]. We observed that mothers with age ≤31 years had positive attitudes towards ITNs [OR=0.461; 95%CI= (0.2578-0.8232)]. In addition, mothers with formal education were more likely to have positive attitude toward ITNs two time higher than those with informal education [OR; 1.99 (95% CI: 1.1182- 3.5731)]. Only association between income and practice is evident. Higher income group is more likely to practice preventive activities two time higher than those with low income group [OR; 1.69 (95% CI: 1.0158-2.8214)]. We Conclude that the attitude and practice of mothers to ITNs in this study was poor. Multivariate analysis revealed that knowledge of mothers about ITNs has significant association with age and education, also illustrate that mothers attitude towards ITNs has significant association with age, mother’s work, education and monthly income. Therefore, Education System and the Malaria Control Programme in North Sudan should work closely, especially on malaria education for behaviour change as a key element for increasing utilization of ITNs.