Journal: |
ULTRASTRUCTURAL PATHOLOGY
Taylor& Francis
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Volume: |
46
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Abstract: |
Exposure to the deleterious effects of silver nanoparticles (AgNPs) is inevitable due to their wide use in
medicine and daily life. The current study aimed to delineate the histomorphological changes and the
molecular mechanisms underlying the ameliorative effect of Resveratrol (RSV) on rats’ livers exposed to
AgNPs. Fifty healthy adult male Wistar albino rats were divided into four groups: control, AgNPsexposed, RSV-treated after AgNPs exposure, and recovery groups. Liver sections were examined by
light and electron microscopes, and immunohistochemistry was performed for detection of activated
caspase3 and TNFα. Serum ALT and AST, plasma levels of TNFα, IL-6, GSH and SOD were measured.
mRNA expression of SIRT1, ADORA3, PAI, CDK1, Nrf2 and NFκB genes in liver tissue homogenate was
performed using qRT-PCR. AgNPs-exposure for 28 days caused marked liver tissue damage with
trapping in hepatocytes and Kupffer cells, while RSV treatment ameliorated liver ultrastructure and
function. Our results clarified the molecular basis of RSV ameliorative effect on liver tissue by significant
upregulation of SIRT1-NrF2 signaling pathway with increased levels of the antioxidant GSH and SOD,
which represent the antioxidant effect of RSV. Significant upregulation of the protective ADORA3 with
downregulation of the proinflammatory PAI-1 and NFκB mRNA expression levels besides decreased
plasma levels of TNFα, IL-6 and decreased immunoexpression of TNFα in liver tissue, represent the antiinflammatory effect of RSV. In addition, decreased immunoexpression of caspase3 and downregulation
of CDK1 expression, represent its antiapoptotic effect. In conclusion: RSV ameliorates AgNPs-induced
liver damage by antioxidant, anti-inflammatory and antiapoptotic effects.
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