Abstract Background: Simple anal fistula is a common surgical condition that could be treated efficiently with fistulotomy or fistulectomy, fistulotomy wounds take around 6 to 7 weeks to achieve complete healing, some studies offered different methods to enhance fistulotomy wound healing, among these methods was marsupialization of the fistulotomy wound edges. This study was carried out to evaluate the effect of marsupialization of fistulotomy wound in simple anal fistula. Patients and methods: 70 patients with simple anal fistula participated in this trial, they were randomly allocated into two groups, group A; underwent fistulotomy alone and group B; underwent fistulotomy with marsupialization of the wound edges, follow up data including postoperative complications and state of wound healing together with preoperative data were recorded and analyzed properly. Results: The demographic data, presenting manifestations and pathological findings showed non-significant differences between both groups, the operative time was significantly shorter in group A( fistulotomy), late postoperative pain after 1 week was significantly better in group B (fistulotomy and marsupialization), no significant differences between both groups in terms of complications (bleeding, incontinence and urine retention), complete wound healing time was significantly shorter in group B (4.8) weeks than group A (6.8) weeks. Conclusion: Marsupialization of the fistulotomy wound edges in simple anal fistula slightly prolongs the operation time but significantly improves postoperative pain and efficiently enhances wound healing time.