This investigation was performed at the Agriculture Research Station, Faculty of Agric. Zagazig Univ., Sharkia Governorate, Egypt, throughout the winter seasons of 2019/ 2020 and 2020/ 2021. The study aimed to evaluate the influence of ten bio-chemical fertilization and lithovit regimes on forage quality of 1st and 3rd cuts of various Egyptian clover varieties (Berseem, Trifolium alexandrinum L.) q.e. Helaly, Gemmeza 1, Sakha 4, Serw 1, Giza 6 and local variety. The ten fertilization regimes were F1, control; F2, chemical phosphorus 15.5 P2O5 Kg fad-1; F3, bio-phosphorus fertilizer ‘phosphorein‘; F4, 50 % of F2 + phosphorein; F5, 25 % of F2 + phosphorien; F6, F2 + lithovit; F7, F3 + lithovit; F8, F4 + lithovit; F9, F5 + lihtovit; F10, sole lithovit. Giza 6 Egyptian berseem variety ranked first in forage quality because of its high content of each CP (%), NFE (%), TDN (%), and DP (%) in the 3rd cut. The local berseem variety ranked the least over the six Egyptian clover varieties with the highest ash (%) in the 1st cut. Other varieties were in between. Allusion to the bio-chemical phosphorus fertilization and lithovit regimes, application of any phosphorus fertilization regimes surpassed the control treatment in most nutritive values. Application of F9 fertilization regime (25 % chemical P + bio-fertilizer ‘phosphorien‘ + nano-fertilizer ‘lithovit‘) tended to produce high forage quality with high content of CP (%) in the 1st cut, TDN (%) in both 1st and 3rd cuts as well as DP (%) in the 1st cut.