Fertilization is one of the main factors influencing yield and grain quality of barely because it participates in numerous metabolic routes. Grain viability play important role in malting industry, field emergence, green forage (sprouted barley) and quality parameters. In this manner, this investigation was designed to study effects of three major elements (N,P,K) and bio- fertilizers on productivity and grain quality of three barley cultivars (Giza123, Giza131 and Giza136). The fertilization regimes were F1, control without fertilization; F2, supplying mineral fertilizers in levels of 45 N,30 P and24 K kg/fad as recommended regime; F3, 50% of F2 + biofertilizers (Azotobacter, phosphorein and potassmage); F4, 25% of F2 + biofertilizersand F5, inoculation with the bio- fertilizers (Azotobacter, phosphorein and potassmage). Results of the combined analysis indicated significant varietal differences in most yield attributes and all viability traits ( germination %, seedling dry weight and vigor index) where, Giza 136 surpassed over the other two cultivars( Giza131 and Giza123) .Withal,Giza123 outbraved significantly on Giza 136 and Giza 131 in plant height and straw yield (kg/fad)The F3 fertilization regime (50% of recommended dose+ bio-fertilizers Azotobacter, phosphorein and Potassmage) gave the higher most value for each of chlorophyll content, plant height, No. of spikes/m2, grain weight/spike, 1000- grain weight, grain and straw yields kg/fad, harvest index and carbohydrate content, seedling dry weight and seedling vigor index, while the highest germination (%) was achieved with both fertilization regimes F2 and F3 .Mean germination time was not affected by fertilization regimes.