This investigation was carried out in the Experimental Farm, Fac. of Agric., Zagazig Univ. Ghazala farm, Sharkia Governorate Egypt, during 2017/2018 and 2018/2019 seasons. The study was designed to investigate the effect of four phosphorus bio-chemical fertilization regimes (F1:100% chemical phosphorus”15.5 kg P2O5”, F2:50% of F1+phosphorein, F3:25% of F1+ phosphorein and F4: sole phosphorein) on the yield and seed quality of three lentil varieties (Giza9, Sina1 and Giza29). Results indicated that, with exception for 1000 seed weight, lentil variety Giza-9 significantly surpassed Sina1 and Giza29 in plant height, No. of branches/plant, No. of pods/plant, seed yield/fad., and biological yield/fad., as well as harvest index. On the other hand, Sina1 recorded the highest 1000 seed weight. Although Giza29 recoded the lowest values in yield and its components, it has the highest seed protein (%) and seedling root length. The maximum plant height (cm), No. of branches/plant, No. of pods/plant and No. of seeds/pod was obtained with suppling 100% chemical phosphorus. Supremacy each of seed index, lentil seed yield /fad, biological yield /fad and harvest index was the result from suppling either % 15.5 kg P2 O5/fad. 100% P or 50 %P+ phosphorein, both fertilization regimes 25% P + phosphorein and sole biofertilizer phosphorein ranked third and fourth. The same trend was noted in protein yield/fad, root length, shoot length as well as seedling vigor index .