Background:Cystic renal masses (CRMs) are prevalent in radiological practice. Although the vast majority of CRMs are simple benign cysts, up to 8–15% may have a complex appearance. Imaging plays an essential role in the identification and characterization of CRMs as well as in grading and follow-up of patients with renal cell carcinoma. Objective: To assess the diagnostic accuracy and agreement of CT and MRI in terms of the Bosniak classification version 2019 (BCv2019). Materials and methods: A prospective multi-institutional study enrolled 63 patients with 67 complicated cystic renal masses(CRMs) discovered during ultrasound examination. All patients underwent CT and MRI scans andhistopathology. Threeradiologists independently assessed CRMs using BCv2019 and assigned Bosniak class to each CRM using CT and MRI. Thefinal analysis included 60histopathologically confirmed CRMs (41 were malignant and 19 were benign). Results: Discordance between CT andMRI findings was noticed in 50% (30/60)CRMs when data were analyzed in terms of theBosniak classes. Of these, 16 (53.3%) were malignant. Based on consensus reviewing, there was no difference in the sensitivity,specificity, and accuracy of the BCv2019 with MRI and BCv2019 with CT (87.8%; 95% CI = 73.8–95.9% versus 75.6%; 95%CI = 59.7–87.6%; p = 0.09, 84.2%; 95% CI = 60.4–96.6% versus 78.9%; 95% CI = 54.4–93.9%; p = 0.5, and 86.7%; 95% CI =64.0–86.6% versus 76.7%; 95% CI = 75.4–94.1%; p = 0.1, respectively). The number and thickness of septa and the presence ofenhanced nodules accounted for the majority of variations in Bosniak classes between CT and MRI. The inter-reader agreement(IRA) was substantial for determining the Bosniak class in CT andMRI (k = 0.66; 95%CI = 0.54–0.76, k = 0.62; 95%CI = 0.50–0.73, respectively). The inter-modality agreement of the BCv219 between CT and MRI was moderate (κ = 0.58). Conclusion: In terms of BCv2019, CT and MRI are comparable in theclassification of CRMs with no significant difference indiagnostic accuracy and reliability.