Background: Symptoms of diabetes affect vision, and high levels of blood glucose draw fluid from tissues, including the lenses of the eyes. The aim of this study is to study the chemical and biological properties of Passion fruit as a protective effect on oxidative stress, blood sugar level, insulin sensitivity, HOMA-index, lipid profile, and cataract formation in alloxan-induced diabetic rats. Methods: Twenty-eight male rats were randomly divided into 4 groups (7 rats in each group). groups were divided into a negative (-ve) control group, positive control (+ve), and 2 groups. All groups were injected with alloxan (120 mg/kg body weight) except a negative (-ve) control group. The first and second groups were fed on a basal diet, while treated groups (3&4) were fed on a basal diet and given orally 2 and 4 ml of concentrated Passion fruit juice/ day, respectively. Results: The results of the analysis of phenolic compounds showed a high amount of rutin, trans-cinnamic, and p-coumaric acids in Passion fruit . After 8 weeks of the biological experiment, the animals treated with Passion fruit juice showed a significant decrease in the level of blood glucose, insulin, BMI, HOMA-index of triacylglycerols, total cholesterol, LDL-C, VLDL-C, Lipid peroxidation in blood and lens, and a significant decrease in the concentration of lens Fas ligand compared with the positive control (+ve). Also, the treated groups showed a significant increase in the level of HDL-C, glutathione ,the activity of superoxide dismutase , and a significant decrease in the levels of nitric oxide concentration. Slit lamp examination revealed that supplementation with levels at 2 and 4 ml of Passion fruit juice delayed the progression and maturation of cataract in the treated groups compared the positive control (+ve). Conclusion: The importance of consuming Passion fruit is due to its preventive effect which reduces the complications of diabetes and the formation of new blood vessels in the retina of the eye. The current study recommends the inclusion of Passion fruit juice in our daily dishes, beverages, and pharmaceutical compounds.