Date palm has been shown to have excellent antioxidant activity as a result of containing high flavonoids and phenolic. It has been cited in several studies for the treatment of liver problems, as well as gastric and intestinal disorders. Aim of the study: The present study was administrated to inspect the hepatoprotective activity of burgers mixed with date palm seeds on carbon tetrachloride (CCl4)-induced Hepatotoxicity in rats. Materials and Methods: Thirty-six rats were divided into two main groups, the first group (n= 6 rats) was G (1): control (-ve), rats of second main group (n= 30 rats) were injected CCl4 twice a week for two weeks to induce hepatotoxication. After that, divided into 5 groups (each 6 rats) as follow: G (2): Hepatotoxicated rats as a control (+ve) and fed on basal diet only. G (3): Injected rats by CCl4 were fed on basal diet with burgers control, G (4, 5 and 6): Injected rats by CCl4 were fed on basal diet with burgers mixed with date palm seeds (10, 20 and 30g /kg), respectively. Results: Results demonstrated that burgers mixed with date seeds revealed significant improvement blood indicaors of CCl4-induced hepatotoxicated rats, hemoglobin (HB) and packed cell volume (PCV), (AST, ALT and ALP) liver function parameters. Also, both serum creatinine and uric acid levels were significantly decreased and nitric oxide (NO). In addition, it restored the antioxidant enzymes activities (glutathione S-transferase (GST), Catalase (CAT) and superoxide dismutase (SOD) that were deficient after CCl4 induction. Conclusions: The Date Palm (Phoenix dactylifera L.) seeds DM might be a promising alternative for CCl4-induced liver intoxication protection, and this hepatoprotective activity could be linked to antioxidant activities. It can be utilized as a natural preservative additive in suggestive formulations as a functional food and dietary supplement through biotechnological processes.