Effects of different this stud designed to investigate the phytochemicals contents and biological concentrations of Annona juice with the objective of inhibition or decreasing uric acid production in hyperuricemia-induced aspirin. Thirty-five adult male albino rats Sprague Dawley strain weighting (130±10g) were randomly classified into five groups (7 rats each). The first group served as a negative control group, fed on basal diet only. The other four groups were fed on basal diet and received oral aspirin 3.5 g/ kg body for one weeks to induce hyperuricemia and reclassified into positive control (untreated) and treated rat groups that were 100,200 and 300 grams of Annona juice respectively. The treatment period is designed for six weeks. The chemical composition of Annona juice showed higher value of Moisture and carbohydrate but lower value of Fat, Ash, Protein and fiber. The Antioxidant activity and Total phenolic higher percent in Annona juice. In compared to positive control group, the treated rats group with Annona juice showed a significant increase in weight gain, food intake and FER and also Total antioxidants and Superoxide dismutase but a significant decrease in serum Uric acid, Urea, Creatinine, ALT &AST, ALP, total bilirubin and, MDA at P< 0.01. Our study clearly demonst1rated that administration of an Annona juice alleviates the harmful effect aspirin induced hyperuricemia in rats