In recent years, red radish has sparked much interest in health and nutrition. The present study aimed to clarify the effect of 1, 2 and 3 ml /Kg /B W/rats red radish juice protective on serum leptin, adiponectin, oxidative stress, and lipid profile to induce obesity in rats. The phenolic content and antioxidant activity of the red radish juice were analyzed. A biological experiment was performed in 36 male rats distributed into 6 equal groups. Group (1) was kept as normal control (fed on a basal diet), while rats of the other five groups were fed on a diet with beef tallow 19% and soybean oil 1% to induce obesity. Rats of the group (2) were left as a positive control and three groups (3, 4 and 5) were given red radish juice (1, 2 and 3 ml/kg /B wt/rats). After 4 weeks, body weight, BMI, FER, leptin, adiponectin, tumor necrosis factor-alpha (TNFα), total antioxidant capacity, total oxidant capacity, and lipid profile were analyzed in the study of animals. Results showed high ferric reducing antioxidant (FRAP) and total radical-trapping antioxidant parameter (TRAP) and phenolic content of the red radish extract. Red radish juice showed a significant decrease in leptin, adiponectin, TNF-α, total oxidant capacity while increasing the total antioxidant capacity in addition to lipid profile normalization in group 5 that received a fat diet containing 3 ml red radish juice/kg /B wt/rats as compared to the negative control group. Therefore, it can be concluded that the consumption of red radish juice as a functional food for delay or protect risk of adipose tissue. As well as a clear improvement in blood analyzes.