Sesame is rich in bioactive food components has therapeutic properties on anemia in rats. The present study aimed to evaluate the effect of sesame seeds and its oil on management of anemia, lipid profile and improve antioxidant status in animal. This study was conducted on twenty albino male rats and categorized into 4 groups (5 rats per each). Group (1) kept as normal group (control -ve), while the other three groups were administered a single dose of phenyl hydrazine injection 40 mg/kg/day for two days, then rats categorized into three groups which were group 2 control positive (+ve) (untreated) and groups (3and 4) treated with 15% as a powder of sesame seeds and 5 ml of oil by stomach tube daily for eight weeks, respectively. The obtained results revealed that sesame seed and oil contain highest amount of phenolic compounds and polyunsaturated fatty acids. The biological results showed that significant (p≤0.05) increase in hemoglobin and RBCs were noticed in rats treated with sesame seeds and oil when compared with positive control> Also, significant (p≤0.05) increase in total antioxidants (TA), superoxide dismutase (SOD), were observed in rats treated with Sesame seeds and oil than positive control. The results showed that a decrease significantly (p≤0.05) in serum triglyceride, total cholesterol and LDL-c, VLDL-c levels and a significant (p≤0.05) increase in HDL-c level were spotted in rats treated with sesame seed and oil than positive control.