Background: Type 2 innate lymphoid cells (ILC2s) are introduced as a key player in type 2 inflammation. Objectives: To assess the correlation between ILC2s in peripheral blood, sputum, and colonic biopsies among patients with asthma, and to assess the relation between ILC2s frequency and severity of asthma. Methods: ILC2s numbers were assessed in peripheral blood, and sputum among 29 patients with asthma (asthma group) and 29 healthy controls (control group). Colonic biopsies were obtained from twelve mild to moderate asthma patients and nine healthy controls. ILC2s were determined by multicolor flow cytometer in all specimens. Results: ILC2s numbers in peripheral blood were higher among the asthma group compared to the control group (P<0.001). ILC2 in peripheral blood and sputum were significantly elevated in severe asthma (P <0.001). There was a strong positive correlation between ILC2s numbers in peripheral blood, and sputum (P<0.001) and severity of asthma. ILC2s were not detected in the colonic biopsy of the controls but was detected in the asthma patients. Conclusion: Type 2 innate lymphoid cells were increased in peripheral blood, sputum, and colonic biopsies among asthma patients, particularly severe asthma, suggesting its role in the pathophysiology of asthma. Clinical implication: The lack of Knowledge regarding relation between ILC2s in blood, sputum and gut mucosa to assess the value of ILC2s as an indicator among patients with asthma,