liver is the main organ in the body for intense metabolism and excretion. A large number of chemicals and medicines/drugs used routinely in daily lives can cause disorders and possibly liver disease. The aim of exploring some aspects related to the potential hepatoprotective activity of Ganoderma lucidum versus carbon tetrachloride (CCl4) intoxication in rat liver. When compared with the normal group rats, the CCl4 treated rats showed significant (p≤0.05) decreased in different biological parameters. Also, biochemical parameters such liver enzymes activities were significant (p≤0.05) elevation when compared with the normal group rats. For immunological parameters, Alb was significant (p≤0.05) decreased by the rate of -29.44 and TNF-α increased by 93.28%. This also coincided with an imbalance in the oxidants/antioxidants status in the blood, which was represented by a decrease in the level of antioxidants and a high level of oxidants. All of those parameters were indicating the liver injury by CCl4. Whereas animal treated/fed with Ganoderma lucidum powder (GLP) showed significant (p≤0.05) improvements in all previous status biomarkers indicating the protection against hepatic cell damage. A positive dose - response was recorded between the concentrations of GLP applied and the level of improvement noticed in all measured markers. In conclusion, GLP was effective in protecting against CCl4-induced liver disorders. Present study recommended like of that algae powder by a concentrations up to 5% (w/w), amount to be included in daily diets, drinks and food supplementation after trial study on volunteer human.