Abstract: Primary dysmenorrhea is a common problem among adolescent girls which can have negative effects on their daily activities. Aim: To evaluate the effect of audio peer educational guidelines on awareness of blind adolescent girls' students regarding primary dysmenorrhea. Subjects and Methods: A quasi-experimental design was utilized in this study that was conducted at Al-Noor and Al-Amal School for the Blind, preparatory and secondary in Beni-Suef City. A purposive sample of 150 blind adolescent girls' students from the previously mentioned settings was used in this study. Three tools were used (pre/posttests): 1) Students’ interviewing questionnaire to assess their knowledge about primary dysmenorrhea, 2) An observational checklist to evaluate students' girls' reported practices regarding primary dysmenorrhea, and 3) Blind students’ satisfaction level questionnaire to appraise satisfaction level on audio peer educational guidelines using among blind adolescent girls. Results: There were significant differences regarding knowledge and their reported practices about primary dysmenorrhea between pre, one month post, and follow-up phases of educational guidelines. Conclusion: The present study concluded that the educational guidelines had a positive effect on improving awareness of blind adolescent girls-students regarding primary dysmenorrhea. Recommendations: Integrating the topic of menstruation, menstrual hygiene, and menstrual sickness management into the course contents of the blind school's objectives.