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Hypoxia pretreatment enhances the therapeutic potential of mesenchymal stem cells (BMSCs) on ozone induced lung injury in rats
Faculty
Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
17
Authors:
Shimaa Ali Abdelrahman
Staff Zu Site
Abstract In Staff Site
Journal:
Cell and Tissue Research Springer
Volume:
389(2)
Keywords :
Hypoxia pretreatment enhances , therapeutic potential of mesenchymal
Abstract:
Ozone (O3) gas is a double-sided weapon. It provides a shield that protects life on earth from the harmful ultraviolet (UV) rays, but ground-level O3 is considered an urban air pollutant. So, a rat model of chronic O3 inhalation was established to assess the biochemical and morphological alterations in the lung tissue and to investigate the ameliorative effects of bone marrow–derived mesenchymal stem cells (BMSCs) with or without hypoxia pre-treatment. Forty-two adult male albino rats were divided into four groups: control, ozone-exposed, normoxic BMSC-treated, and hypoxic BMSC-treated groups. Lung tissue sections were processed for light and electron microscope examination, immunohistochemical staining for caspase 3, and iNOS. Quantitative real-time PCR for IL-1α, IL-17, TNF-α, and Nrf2 mRNA gene expression were also performed. Chronic O 3 exposure caused elevated inflammatory cytokines and decreased antioxidant Nrf2 mRNA expression. Marked morphological alterations with increased collagen deposition and elevated apoptotic markers and iNOS were evident. BMSC treatment showed immunomodulatory (decreased inflammatory cytokine gene expression), antioxidant (increased Nrf2 expression and decreased iNOS), and anti-apoptotic (decreased caspase3 expression) effects. Consequently, ameliorated lung morphology with diminished collagen deposition was observed. Hypoxia pretreatment enhanced BMSC survival by MTT assay. It also augmented the previously mentioned effects of BMSCs on the lung tissue as proved by statistical analysis. Lung morphology was similar to that of control group. In conclusion, hypoxia pretreatment represents a valuable intervention to enhance the effects of MSCs on chronic lung injury
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Shimaa Ali Abdelrahman, "Effect of Human Umbilical Cord Blood Mesenchymal Stem Cells Administered by Intravenous or Intravitreal Routes on Cryo- Induced Retinal Injury", Wiley, 2017
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Shimaa Ali Abdelrahman, "Effect of Low Frequency Noise on Fundic Mucosa of Adult Male Albino Rats and the Role of Vitamin E Supplementation (Histological and Immunohistochemical Study)", OMICS publishing group, 2015
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Shimaa Ali Abdelrahman, "Morphological alterations in the jejunal mucosa of aged rats and the possible protective role of green tea", via medica, 2017
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