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The Preservative Action of Protein Hydrolysates from Legume Seed Waste on Fresh Meat Steak at 4 °C: Limiting Unwanted Microbial and Chemical Fluctuations
Faculty
Agriculture
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Ahmed Mohamed Saad Mousttafa
Staff Zu Site
Abstract In Staff Site
Journal:
Polymers MDPI
Volume:
Keywords :
, Preservative Action , Protein Hydrolysates from Legume
Abstract:
Valorizing agricultural wastes to preserve food or to produce functional food is a general trend regarding the global food shortage. Therefore, natural preservatives were developed from the seed waste of the cluster bean and the common bean to extend the shelf life of fresh buffalo meat steak and boost its quality via immersion in high-solubility peptides, cluster bean protein hydrolysate (CBH), and kidney bean protein hydrolysate (RCH). The CBH and the RCH were successfully obtained after 60 min of pepsin hydrolysis with a hydrolysis degree of 27–30%. The SDS-PAGE electropherogram showed that at 60 min of pepsin hydrolysis, the CBH bands disappeared, and RCH (11–48 kD bands) nearly disappeared, assuring the high solubility of the obtained hydrolysates. The CBH and the RCH have considerable antioxidant activity compared to ascorbic acid, antimicrobial activity against tested microorganisms compared to antibiotics, and significant functional properties. The CBH and the RCH (500 µg/mL) successfully scavenged 93 or 89% of DPPH radicals. During the 30-day cold storage (4 °C), the quality of treated and untreated fresh meat steaks was monitored. Protein hydrolysates (500 g/g) inhibited lipid oxidation by 130–153% compared to the control and nisin and eliminated 31–55% of the bacterial load. The CBH and the RCH (500 µg/g) significantly enhanced meat redness (a* values). The protein maintained 80–90% of the steak’s flavor and color (p < 0.05). In addition, it increased the juiciness of the steak. CBH and RCH are ways to valorize wastes that can be safely incorporated into novel foods.
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Department Related Publications
Sabah Mohamed Monir, "Impact of instant controlled pressure drop treatment on dehydration and rehydration kinetics of green moroccan pepper (Capsicum annuum)", فرنسا, 2012
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Amal Ahmed Mohamed Mahmoud Adawy, "IMPACT OF TECHNOLOGICAL GAP ON THE CRAFT OF READY-MADE CLOTHES IN RURAL OF SHARKIA GOVERNORATE", مجلة الإنتاجية والتنمية (بحوث زراعية), 2011
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Amal Ahmed Mohamed Mahmoud Adawy, "PROBLEMS WHICH FACE THE READY-MADE CLOTHING IN SOME VILLAGES OF SHARKIA AND HOW TO OVERCOME THEM", مجلة حوليات العلوم الزراعية بمشتهر كلية الزراعة – جامعة بنها, 2011
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Galal Abdallah Galal Abedullah, "مستخلص الكرات المصري كمضاد أكسدة طبيعي: تطبيق علي أقراص اللحم (البيف برجر)", كلية الزراعة - جامعة الأسكندرية, 2013
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