Chewing lice comprise a large group of ectoparasites that colonize and adversely affect several domestic and wild birds including pigeons. In Saudi Arabia, there is a lack of studies describing such ectoparasites and their infestation rates. Through this work, a new record, Columbicola, tschulyschman Eichler (C.tschulyschman Eichler) was collected from domestic pigeons (Columba livia domestica, Linnaeus). The collected C. tschulyschman Eichler was morphologically identiied based on speciic taxonomic keys.Mitochondrial (COI) and nuclear (EF-1α) gene fragments were used for molecular identiication and phylogenetic reconstruction. In this study, the C. tschulyschman Eichler accounted for around 69.40%. To our knowledge, this is the irst report of C.tschulyschman Eichler in Riyadh, Saudi Arabia. To improve the tree topology and differentiate between genera, further studies should utilize the 16s rRNA.