The expulsion of iron from water is an essential issue. Exceeding iron concentrations in water, it become more toxic and cause several troubles for human health and environment. The biosorption is the upcoming mechanism to treat the iron from wastewater. Microorganisms perform an important function in the bioremediation of wastewater. This study was conducted to investigate the removal of iron by dried biomass of Aspergillus niger (A.niger). The dried A. niger was tested as a sorbent for the removal of iron from wastewater. The effects of various experimental parameters as initial iron concentration, amount of biomass, contact time and the initial pH solution were examined and optimal experimental conditions were obtained. The obtained adsorption results were fitted to the Langmuir, Freundlich, and Temkin equations. The study showed that dried A. Niger biomass in high concentration 4 g/100 ml was found to be more effective in the removal of iron from water at pH 3 with contact time 60 min. the A. Niger successfully removed iron and has ability to be regenerated and reused in the removal process.