This current study investigated the effect of probiotics and prebiotics on the control of Salmonella spp. isolated from chicken. One hundred and eleven bacterial isolates were recovered from different chicken farms, and nine Salmonella isolates were detected with 8%. The serogroup analysis of nine Salmonella isolates showed three different groups identified as (4) S. enteridits, (3) S. typhimurium, and (2) un-typed group. The positive-identified Salmonella was using PCR and genus-specific primer OMPCF (outer membrane protein reverse) with a target size of 204bp. The results of Salmonella enteridities with target size are also 304bp and target 401bp for Salmonella typhimurium. In addition, one hundred chicks were grouped into five groups (1, 2, 3, 4& 5) containing 20 broiler chicks. The broilers of groups 1, 2, 3, 4 & 5 were orally inoculated with a dose of 1 x 108 cfu nalidixic acid-resistant Salmonella enteridities. Experimental groups were as follows: Group 1: negative control, Group 2: positive control (infected chickens), Group 3: infected chickens and treated with B. subtilus probiotic, Group 4: infected chickens and treated with levoxyl prebiotic. Group 5: infected chickens and treated with panflor antibiotic.. Results showed significantly increased survival percentage against those challenged with a virulent strain of nalidixic acid probiotics and prebiotics. The oral application significantly improved the survival percentage against challenges with a virulent strain of nalidixic acid-resistant Salmonella spp. The best result of B. subtilis was 1x 108 which reduced the growth of the microorganism under study (S. typimurium - S.