To determine the distribution pattern of the threshing and separating device, the simulation experiment on the distribution pattern of our self-designed drum-shape bar-tooth longitudinal axial flow threshing and separating device was carried out with the help of the EDEM software, by which the axial and radial distribution curve of the threshed mixture along the cylinder was acquired. The three-dimensional distribution of the mass of the threshed mixture was drawn by using the Matlab software, and the bench test was carried out on the self-built small-scale longitudinal axial flow threshing cylinder performance test platform, which was consistent with the simulation conditions. The results showed that the axial and radial distribution of the threshed mixture was uneven, and the axial distribution of the threshed mixture decreased gradually, which was mainly distributed in the first third section of the cylinder. The distribution of the threshed mixture along the radial area of the cylinder was gradually decreasing at first and then increasing, i.e., the total mass of the threshed mixture on the left and right sides was higher than that of the middle area, which was basically consistent with the simulation results. The research can provide reference for the optimization of structural parameters of threshing and separating device and cleaning system.