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Antibacterial activity of bioactive compounds extracted from red kidney bean (Phaseolus vulgaris L.) seeds against multidrug-resistant Enterobacterales
Faculty
Veterinary Medicine
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Norhan Khayry Abdelaziz Mahmoud
Staff Zu Site
Abstract In Staff Site
Journal:
Frontiers in Microbiology Frontiers
Volume:
Keywords :
Antibacterial activity , bioactive compounds extracted from red kidney
Abstract:
In the present study, biologically active compounds such as phenolic-rich extract (PRE), 7S globulin (vicilin), and 11S globulin (legumin) from red kidney bean (Phaseolus vulgaris L.) seeds were extracted and evaluated as antibacterial agents against multidrug-resistant (MDR) Enterobacterales isolated from both animal and human sources. The overall occurrence rate of Enterobacterales was 43.6%, which significantly differed between animal (38.75%) and human (56.67%) sources. Antimicrobial susceptibility testing revealed that Enterobacterales isolates exhibited full resistance (100%) to amoxicillin-clavulanic acid, followed by ampicillin (75.44%), erythromycin (71.93%), cefoxitin (70.18%), amoxicillin (66.66%), ceftriaxone (64.91%), and trimethoprim/sulfamethoxazole (56.14%). Worthy of note, 97.92% of Enterobacterales isolates were MDR. The total phenolic contents (TPC; 53 2 mg GAE g-1) and total flavonoid contents (TFC; 26 1 mg QE g-1) were recorded. The major phenolic and flavonoid components were catechol (17.63 mg/mL) and hesperidin (11.37 mg/mL), respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to detect the 7S and 11S globulin‘s molecular mass. The data revealed that red kidney bean protein isolate (KPI) includes two major portions: 7S and 11S globulins. The bioactive compounds of Phaseolus vulgaris were investigated for their antibacterial activities against Enterobacterales for the first time. The protein component (MIC = 0.125 – 2 mg/mL; 53.85%) and its 7S and 11S globulin subunits (MIC = 0.5 – 2 mg/mL; 30.77% each) were the mospotent extracts, whereas the methanolic extract was the least effective one (MIC = 2 mg/mL; 15.38%). The results displayed the potential of protein bioactive compounds as a hopeful candidate for enhancing future medication plans for the treatment of Enterobacterales originating from animal and human sourcest
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Norhan Khayry Abdelaziz Mahmoud, "Mutations of domain V in 23S ribosomal RNA of macrolide-resistant Mycoplasma gallisepticum isolates in Egypt", Open Learning on Enteric Pathogens, 2016
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Norhan Khayry Abdelaziz Mahmoud, "Propidium Monoazide Quantitative Real-Time Polymerase Chain Reaction for Enumeration of Some Viable but Nonculturable Foodborne Bacteria in Meat and Meat Products", Mary Ann Liebert, Inc., 2018
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Norhan Khayry Abdelaziz Mahmoud, "Class 1 integron and associated gene cassettes mediating multiple-drug resistance in some food borne pathogens", Malaysia, 2016
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Norhan Khayry Abdelaziz Mahmoud, "حلقة نصاب الاستشعار الهرمى المعقد تنظم التعبير الجينى للفينازين فى السيدوموناس ايروجينوزا", The Journal of Infection in Developing Countries, 2016
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Norhan Khayry Abdelaziz Mahmoud, "تلازم محددات المقاومة للكينولون بواسطة البلازميد و AmpC بيتا لاكتاماز فى عترات الاشرشيا كولاى فى مصر", Cellular and Molecular Biology, 2016
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Department Related Publications
Mohammed Mahmoud Alswedy , "Effect of dietary constituents on plasma lipoprotein cholesterol and triglycerides in rats", لايوجد, 1900
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Mohammed Mahmoud Alswedy , "Effect of some clinically used anions in assays of total and ionized calcium in serum", لايوجد, 1900
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Mohammed Mahmoud Alswedy , "Action of Antidepressant and Antipsycotic drugs on some biochemical parameters in rabbits ", لايوجد, 1900
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