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Anti-biofilm potential of Lactobacillus plantarum Y3 culture and its cell-free supernatant against multidrug-resistant uropathogen Escherichia coli U12
Faculty
Science
Year:
2022
Type of Publication:
ZU Hosted
Pages:
Authors:
Fifi Mohamed Reda Mohamed
Staff Zu Site
Abstract In Staff Site
Journal:
Saudi Journal of Biological Sciences Elsevier
Volume:
Keywords :
Anti-biofilm potential , Lactobacillus plantarum , culture , , cell-free
Abstract:
Uropathogens develop biofilms on urinary catheters, resulting in persistent and chronic infections that are associated with resistance to antimicrobial therapy. Therefore, the current study was performed to control biofilm-associated urinary tract infections through assaying the anti-biofilm ability of lactic acid bacteria (LAB) against multidrug-resistant (MDR) uropathogens. Twenty LAB were obtained from pickles and fermented dairy products, and screened for their anti-biofilm and antimicrobial effects against MDR Escherichia coli U12 (ECU12). Lactobacillus plantarum Y3 (LPY3) (MT498405), showed the highest inhibitory effect and biofilm production. Pre-coating of a microtitre plate with LPY3 culture was more potent than co-incubation. Pre-coating with LPY3 culture generated a higher anti-biofilm effect with an adherence of 14.5% than cell free supernatant (CFS) (31.2%). Anti-biofilm effect of CFS was heat stable up to 100 °C with higher effect at pH 4–6. Pre-coating urinary catheter with LPY3 culture reduced the CFU/cm2 of ECU12 attached to the catheter for up to seven days. Meanwhile, CFS reduced the ECU12 CFU/cm2 for up to four days. Scanning electron microscope confirmed the reduction of ECU12 adherence to catheters after treatment with CFS. Therefore, Lactobacillus plantarum can be applied in medical devices as prophylactic agent and as a natural biointervention to treat urinary tract infections.
Author Related Publications
Fifi Mohamed Reda Mohamed, "Toxicological Studies and Field Applications of a New Bacillus thuringiensis isolate (Bt1) and Two Chemical Pesticides on Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae).", egypt, 2016
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Fifi Mohamed Reda Mohamed, "Efficient Inhibition of Some Multi-Drug Resistant Pathogenic Bacteria by Bioactive Metabolites from Bacillus amyloliquefaciens S5I4 Isolated from Archaeological Soil in Egypt1", Springer, 2016
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Fifi Mohamed Reda Mohamed, "Detoxification of enterotoxigenic Bacillus cereus (JX455159) isolated from meat by a local strain of Lactobacillus plantarum (JX282192)", springer, 2013
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Fifi Mohamed Reda Mohamed, "Study the biological activity of spores and supernatants of some actinomycetal and bacterial isolates on certain aspects of Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae).", Egypt, 2013
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Fifi Mohamed Reda Mohamed, "Synergistic effect of combination treatment by certain plant extracts and some antibiotics on the resistance of pathogenic bacteria to some common antibiotics", life sciencesite, 2013
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Department Related Publications
Ashwak Mohamed Foad Abotaleb, "استخدام سلسله تفاعل البلمره المتسلسل مع زوج واحد من البادئات لتشخيص تفطر الدم المسبب بثلاثة انواع من الفطور في الاطفال حديثى الولادة", لايوجد, 1900
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Ayman Abdelrahman Hussinn, "الخصائص السريرية و الميكروبية لعدوى المستشفيات التى تسببها انواع البكتيريا الراكدة", لايوجد, 1900
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Hanan Mohamed Elsayed Ibrahiem, "Diagnosis of Acute Bacterial Endophthalmitis By Nested Polymerase Chain Reaction", لايوجد, 1900
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Hanan Mohamed Elsayed Ibrahiem, "Chlamydia trachomatis detection by polymerase chain reaction (PCR) in infertile women using a tubal cytobruch during laparoscopy. 1 /11/1999 Zagazig University Medical J ", لايوجد, 1900
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Alaaeldeen Abdelbaset Hadhoud, "Plasmid profile analysis of tmp resistance negative bacilli among patients unit", لايوجد, 1900
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